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Thermo Scientific Evolution 300 UV-Vis Spectrophotometer

For over 60 years, customers in world-renowned research institutions, highly regulated QC laboratories and teaching
institutions have relied on Thermo Scientific™ UV-Visible (UV-Vis) spectrophotometers. The Thermo Scientific™ Evolution™ 300 UV-Visible spectrophotometer is founded on the successful legacy of the Pye Unicam, Bausch & Lomb™, Phillips Scientific, and Thermo Scientific™ SPECTRONIC™ products. Many customers today say they made their first absorbance measurement on a Thermo Scientific SPECTRONIC 20 spectrophotometer, a product introduced to the market in 1953. The Evolution 300 continues in this tradition by delivering the high level of performance, accuracy and precision you need for your UV-Vis applications.

Specifications

Optical DesignModified Ebert
Double beam with sample and reference cuvette/accessory positions
Spectral BandwidthsVariable 0.5, 1.0, 1.5, 2.0, 4.0 nm
Light SourceXenon Flash Lamp
Typical Lifetime: >5 years. Longer if not using live signal.
Warranty Period: 3 year source replacement warranty
DetectorDual Matched Silicon Photodiodes
GratingHolographic, 1200 lines/mm, blazed at 240 nm
Beam Separation210 mm
Scan Ordinate ModesAbsorbance, % Transmittance, % Reflectance, Concentration,
1st–4th Derivative
WavelengthRange190–1100 nm
Accuracy±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm
RepeatabilityStandard deviation of 10 measurements <0.05 nm
Scanning Speeds3800, 2400, 1200, 600, 240, 120, 60, 30, 10, 5, 1 nm/min
Intelliscan
Data Interval10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm
PhotometricRange>4 A
Display±6 A
ReadoutAbsorbance, % Transmission, % Reflectance, Concentration
Accuracy – Instrument11A: ±0.004 A
2A: ±0.004 A
3A: ±0.006 A
Accuracy – Including Standard Tolerance21A: ±0.008 A
2A: ±0.010 A
3A: ±0.018 A
Noise0A: <0.00018 A
1A: <0.00022 A
2A: <0.00050 A
500 nm, 2.0 nm SBW, RMS
Repeatability1A: ±0.0025 A
Drift<0.0005 Abs/hour, 500 nm, 2.0 nm SBW, 2 hr warm-up
Stray Light198 nm: 2.9 A KCl
220 nm: 4.2 A NaI
340 nm: 4.3 A NaNO2
Baseline Flatness±0.0015 A (200–800 nm)
2.0 nm SBW, smoothed
Dimensions (W × D × H)61 × 53 × 38 cm
Weight22 kg
Electrical Supply100–240 V, 50–60 Hz
Pharmacopoeia Compliance Testing
Resolution (Toluene in Hexane)Peak/Trough Ratio >2.0
Photometric Accuracy (60mg/L K2Cr2O7)<±0.01 A relative to calibrated sealed standard
Stray Light<0.13 %T at 198 nm with KCl per EP
220 nm: <0.01%T NaI (behaves identically to KI for USP)
Wavelength Accuracy±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm
Wavelength RepeatabilityPeak separation of repetitive scanning of Hg line source <0.10 nm

Product Information PDF

Evolution 300
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