Nanodrop Lite, Nanodrop 8000 & Nanodrop 3300 Fluorospectrometer
Rely on fast, accurate quantification of DNA, RNA and protein samples using only 1–2 μL with Thermo Scientific™ NanoDrop™ microvolume instruments. No dilutions needed even for highly concentrated samples with pioneering sample-retention technology* using optical measurement pedestals. For over 15 years and with over 40,000 citations, NanoDrop instruments have been helping scientists around the world do their best work. With preconfigured methods for common life science applications NanoDrop instruments make a novice perform like an expert. It’s as simple as pipette, measure, know!
NanoDrop instrument specifications
| Description | NanoDrop One/OneC Spectrophotometer | NanoDrop Lite Spectrophotometer | NanoDrop 8000 Spectrophotometer | NanoDrop 3300 Fluorospectrometer |
|---|---|---|---|---|
| Instrument Control | Built-in touchscreen | Local control | Computer software | Computer software |
| Minimum Sample Size | 1 μL | 1 μL | 1 μL | 1 μL |
| Sample Number | 1 | 1 | up to 8 | 1 |
| Pathlength(s) | 0.030 to 1.0 mm auto-ranging | 0.5 mm | 1.0 to 0.2 mm, auto-ranging | N/A |
| Light Source(s) | Xenon flash lamp | Light emitting diodes | Xenon flash lamp | Light emitting diodes |
| Excitation Maxima of LEDs | N/A | N/A | N/A | UV: 365 nm, Blue: 470 nm, White: 460–650 nm |
| Detector Type | 2048-element CMOS linear image sensor | Silicon photodiode | 2048-element linear silicon CCD array | 2048-element linear silicon CCD array |
| Wavelength Range | 190–850 nm | 260 and 280 nm | 220–750 nm | 400–750 nm |
| Wavelength Accuracy | ±1 nm | N/A | ±1 nm | ±1 nm |
| Spectral Resolution | ≤1.8 nm (FWHM at Hg 254 nm) | <8.0 nm | 3 nm (FWHM at Hg 546.1 nm) | 8.0 nm (FWHM at Hg 546.1 nm) |
| Typical Measurement Repeatability | 0.002 A (1.0 mm path) or 1%CV, whichever is greater | 0.002* | 0.003* | <5% CV (10 nM fluorescein) |
| Absorbance Accuracy** | 3% (at 0.97 A, 302 nm) | 3% (at 1.05 Abs at 260 nm) | 3% (at 0.74 Abs at 350 nm) | N/A |
| Absorbance Range (10 mm equivalent) | Pedestal: 0–550A, Cuvette: 0–1.5A | 0–30 Abs | 0–75 Abs | N/A |
| Lower Limit of Detection | Pedestal: 2 ng/μL (dsDNA) 0.06 mg/mL (BSA) Cuvette: 0.2 ng/μL (dsDNA) 0.006 mg/mL (BSA) | 4 ng/μL (dsDNA) 0.12 mg/mL (BSA) | 2.5 ng/μL (dsDNA) 0.15 mg/mL (BSA) | <1 fmol fluorescein |
| Maximum Concentration | Pedestal: 27,500 ng/μL (dsDNA) 820 mg/mL (BSA) | 1,500 ng/μL (dsDNA) 45 mg/mL (BSA) | 3,700 ng/μL (dsDNA) 100 mg/mL (BSA) | N/A |
| Measurement and Data Processing Time | 8 seconds | <5 seconds | <20 seconds | 2–10 seconds |
| Footprint | 20 × 25.4 cm | 16 × 11.5 cm | 24 × 32 cm | 14 × 20 cm |
| Weight | 3.6 kg | 0.8 kg | 3.4 kg | 1.5 kg |
| Sample Pedestal | 303 stainless steel and quartz fiber | 303 stainless steel and quartz fiber | 303 stainless steel and quartz fiber | 303 stainless steel and quartz fiber |
| Cuvette Position | Optional (with stirring) | N/A | N/A | N/A |
| Operating Voltage | 12 V (DC) | 6 V (DC) | 12 V (DC) | 5 V (DC) |
| Operating Power Consumption | 12–18 W | 18 W | 30 W | 2 W |
| Standby Power Consumption | 5 W | <2.5 W | 6 W | 1 W |
| Software Compatibility | Standalone control. PC Viewer: Windows® 7 and 10 Professional (64 bit) | Standalone control with data export via USB Flash Drive | Windows® 7 and 10 Professional (64 bit) | Windows® 7 and 10 Professional (64 bit) |
| Advanced Connectivity | NanoDrop One Cloud Application*** | N/A | N/A | N/A |