Protein Quantitation Guide
Highly referenced kits you can trust
Our ELISA kits have been referenced in over 3,000 publications and our multiplex assays cited in over 1,000 publications.
We offer a wide range of immunodetection-based products—in convenient, ready-to-use formats for sensitive, specific detection of intracellular or extracellular proteins. We have developed antibody pair kits, enzyme-linked immunosorbent assay (ELISA) kits for single-analyte analysis, and multiplex assays for multi-analyte analysis. Our kits undergo a rigorous validation process for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency, helping to enable dependably accurate results. You can use our Invitrogen™ and Thermo Scientific™ immunoassays to investigate any of these popular specific areas and more:
Table 4. Specifications for kit development.
|Benchmarking to ELISA||Correlates with ELISA data|
|Recovery||Tested on serum and plasma|
|Sensitivity*||Physiologically relevant levels, <10 pg/mL (based on detectable signal >2 SD above background)|
|Precision||Inter-assay CV: (between assays)
Intra-assay CV: (within assays
|Specificity||Cross-reactivity tests are performed with other analytes and antibodies|
|Linearity of dilution||High coefficient of correlation between sample dilutions and expected concentration over the range
of the assay
|Parallelism to natural samples||Recombinant standards are compared to natural samples to ensure equivalency|